Journal: Planta

Article Title: A chromosome-scale reference genome and integrative transcriptome provide insight into tissue- and stress-specific responses in tetraploid sainfoin (Onobrychis viciifolia)

doi: 10.1007/s00425-026-05021-y

Figure Lengend Snippet: Transcriptome sampling and tissue-specific gene expression patterns. a Principal component analysis (PCA) of RNA-Seq libraries from 9 tissues. Samples cluster by tissue type, with clear separation between root (RT, RC), flower (FB, OF), stem, leaves (DL, EL, 1st L, and 3rd L). b Flavonoid to condensed tannin biosynthetic pathway with enriched enzymes highlighted. Enzymes encoded by genes identified as tissue-enriched in sainfoin are highlighted in green (young leaves), blue (stem), and grey (roots). PAL phenylalanine ammonia-lyase, C4H cinnamate-4-hydroxylase, 4CL 4-coumarate:CoA ligase, CHS chalcone synthase, CHI chalcone isomerase, F3H flavanone 3-hydroxylase, F3′5’H flavonoid 3’, 5’-hydroxylase, DFR dihydroflavonol reductase, ANS anthocyanidin synthase, ANR anthocyanidin reductase, LAR leucoanthocyanidin reductase, OMT O-methyltransferase, UGT UDP-glycosyltransferase

Article Snippet: In the case of RNA-Seq, stranded libraries were prepared using Novogene’s RNA-Seq library construction workflow and sequencing (150 bp paired-end reads) was carried out on an Illumina NovaSeq 6000 platform.

Techniques: Sampling, Gene Expression, RNA Sequencing

Journal: Planta

Article Title: A chromosome-scale reference genome and integrative transcriptome provide insight into tissue- and stress-specific responses in tetraploid sainfoin (Onobrychis viciifolia)

doi: 10.1007/s00425-026-05021-y

Figure Lengend Snippet: Transcriptomic responses to drought and waterlogging stress in sainfoin. a Principal component analysis (PCA) of RNA-Seq samples from leaves derived from control (C), drought (D), and waterlogged (W) plants. b Volcano plots showing differentially expressed genes (DEGs) between drought vs. control (left) and waterlogging vs. control (right). Genes with log₂ fold-change > 1 and adjusted P -value < 0.05 are highlighted in red. c Venn diagrams showing overlap of up-regulated and down-regulated DEGs between drought and waterlogging treatments. Substantial overlap suggests a shared transcriptional response to both types of water stress

Article Snippet: In the case of RNA-Seq, stranded libraries were prepared using Novogene’s RNA-Seq library construction workflow and sequencing (150 bp paired-end reads) was carried out on an Illumina NovaSeq 6000 platform.

Techniques: RNA Sequencing, Derivative Assay, Control

Journal: mAbs

Article Title: 2D NMR assessment of structural consistency and functional relationships in monoclonal antibodies

doi: 10.1080/19420862.2026.2672774

Figure Lengend Snippet: 2D NMR structural comparison and principal component analysis of the mAb1 aglycosylation series. (A) Spectral overlay of the 2D NMR fingerprints of mAb1-WT and mAb2 illustrating structural differences arising from primary sequence variation. (B) PCA scores plot of processed 2D NMR spectra including the mAb1 co-mix series (0–100% NGHC) and mAb2. PCA was performed using the BioHOS workflow implemented in Mnova (mestrelab research) following spectral normalization, binning, and mean-centering as described in the methods section. The first two principal components capture the majority of spectral variance, with mAb2 clearly separated from the mAb1 samples. (C) Spectral overlay of mAb1-WT and mAb1-N297G (100% aglycosylated), illustrating spectral differences associated with complete loss of Fc N-linked glycosylation. (D) PCA scores plot of the mAb1 co-mix series as a function of increasing NGHC content. Individual points correspond to single spectra from each sample. Spectra containing up to ~25% NGHC occupy a similar region of PCA space, whereas higher NGHC levels progressively diverge along the principal component axes.

Article Snippet: PCA was performed using the BioHOS workflow implemented in Mnova (mestrelab research) following spectral normalization, binning, and mean-centering as described in the methods section.

Techniques: Comparison, Sequencing, Glycoproteomics